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LiquidSEQ™
Test CodeLIQSEQ or 14308
CPT Codes
81464
Preferred Specimen
10 mL whole blood collected in each of two separate Streck cell-free DNA blood collection tubes
Minimum Volume
10 mL
Instructions
This test requires two Streck tubes. Blood will be collected in office via Quest-LiquidSEQ Gen Kit. Specimens may be shipped separately and at different times. Do not hold blood specimen due to short stability. Do not reject.
Blood collection using a straight needle is preferred. Use kit only. All tubes must be filled completely. Gently invert tube at least 8 times immediately after draw.
Blood collection using a straight needle is preferred. Use kit only. All tubes must be filled completely. Gently invert tube at least 8 times immediately after draw.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Do not reject, send to laboratory for testing assessment
Methodology
Next Generation Sequencing
Setup Schedule
Sun, Tues, Thurs
Report Available
7-10 days
Limitations
**This test is not available for New York and Wyoming patient testing**
Reference Range
See Laboratory Report
Clinical Significance
The 523-gene comprehensive genomic profiling panel is intended to help oncologists deliver precision medicine by providing personalized genomic analysis of a patient’s blood. This comprehensive panel offers the opportunity for a broader scope of genomic information compared to single gene tests or smaller panels and an alternative specimen type for comprehensive genomic profiling of a patient’s solid tumor. For example, it may be useful for patients with limited or ill-defined treatment options and for those considering clinical trials in the event tumor tissue is not available or insufficient for tissue-based testing. This panel uses next-generation sequencing to detect mutations present in circulating tumor DNA (ctDNA) extracted from a solid tumor patient’s blood plasma. The test is designed to detect single nucleotide variants (SNVs) and small insertions/deletions, as well as whole-gene copy number alterations and translocations in a select group of genes. Microsatellite instability (MSI) and tumor mutation burden (TMB) are also evaluated.
Performing Laboratory
med fusion

