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QuestAssureD™ for Infants, 25-Hydroxyvitamin D : 91935
Test CodeVTDINF or 91935
Alias/See Also
VIT D, Infants
CPT Codes
82306
Includes
Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2
Instructions
Fasting specimens are preferred but not required.
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST®) at room temperature, centrifuge and remove from the gel within 48 hours.
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST®) at room temperature, centrifuge and remove from the gel within 48 hours.
Transport Container
Preferred Specimen
0.5 mL serum
Minimum Volume
0.2 mL
0.5 mL serum
Minimum Volume
0.2 mL
Transport Temperature
Room temperature.
Specimen Stability
Room temperature: 7 days; Refrigerated: 7 days; Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis; Grossly lipemic; Plasma; Serum not separated from Serum Separator Tube (SST®); Serum not separated from gel or clot within 48 hours; Grossly icteric
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Monday - Saturday
Report Available
2-3 days
Reference Range
Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Clinical Significance
While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.
This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.
This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.
Performing Laboratory
med fusion
