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Parvovirus B19 DNA, Qualitative, Real‐Time PCR : 34296
Test CodePVPQL or 34296
CPT Codes
87798
Transport Container
Preferred Specimens
1 mL (Minimum 0.3 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.3 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.3 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®)
1 mL (Minimum 0.3 mL) amniotic fluid collected in a sterile, leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue
1 mL (Minimum 0.3 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.3 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.3 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®)
1 mL (Minimum 0.3 mL) amniotic fluid collected in a sterile, leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue
Transport Temperature
Refrigerated.
Frozen Fresh Tissue: Transport Frozen
Frozen Fresh Tissue: Transport Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
All other specimens
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
All other specimens
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Tuesday & Friday
Report Available
2-5 days
Reference Range
Not Detected
Clinical Significance
Parvovirus B19 DNA, Qualitative, Real‐Time PCR ‐ Parvovirus B19 is the cause of an infection known as "Fifth Disease". This infection affects children primarily and causes a rash on the face, trunk, and limbs. Joint pain and swelling is more common in adults. Although a portion of those affected have only mild disease, patients with sickle cell anemia, or similar types of chronic anemia, can suffer from severe acute anemia. Infection during pregnancy can lead to severe complications for the fetus. Infections iimmunocompromised patients may lead to serious complications including nephropathy, myocarditis, and persistent anemia. Although serologic detection of circulating antibodies (Parvovirus B‐19 IgM & IgG) is useful to determine recent exposure to the virus, DNA testing provides the most reliable evidence of a persistent infection. Quantitative results of Parvovirus B19 viral load may be useful in determining the response to therapy (usually IVIG) and may be beneficial in assessing the risk for nosocomial transmission.
Performing Laboratory
med fusion
