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Herpes Simplex Virus, Type 1&2, Qualitative, Real‐Time PCR : 34257
MessagePlease order NTC 90569 for HSV 1 and 2 testing from PAP vial (test code HSVPAP).
Test Code
HSVPQL or 34257
CPT Codes
87529x2
Includes
HSV 1 and HSV 2
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in Serum separator tubes (SST). Transfer serum into sterile leak‐proof container and store refrigerated or frozen.
CSF: Collect in a sterile leak‐proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label)
Bronchial wash and BAL: Collect in a sterile leak‐proof container.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in Serum separator tubes (SST). Transfer serum into sterile leak‐proof container and store refrigerated or frozen.
CSF: Collect in a sterile leak‐proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label)
Bronchial wash and BAL: Collect in a sterile leak‐proof container.
Transport Container
Preferred Specimens
1 mL (Minimum 0.3 mL) CSF, serum, pleural fluid, pericardial fluid, amniotic fluid collected in a sterile leak‐proof container.
1 mL (Minimum 0.3 mL) Whole blood collected in: EDTA (lavender‐top) tube, Plasma collected in EDTA (lavender‐top) tube or PPT tube.
Swab collected in a viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label).
0.5 mL vitreous fluid collected in a sterile leak‐proof container.
Alternative Specimens
Bronchoalveolar lavage or bronchial wash.
1 mL (Minimum 0.3 mL) CSF, serum, pleural fluid, pericardial fluid, amniotic fluid collected in a sterile leak‐proof container.
1 mL (Minimum 0.3 mL) Whole blood collected in: EDTA (lavender‐top) tube, Plasma collected in EDTA (lavender‐top) tube or PPT tube.
Swab collected in a viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label).
0.5 mL vitreous fluid collected in a sterile leak‐proof container.
Alternative Specimens
Bronchoalveolar lavage or bronchial wash.
Transport Temperature
Refrigerated.
Stored Frozen specimens: Transport Frozen.
Stored Frozen specimens: Transport Frozen.
Specimen Stability
APTIMA® Unisex Collection device (white label)
Room temperature: 14 days; Refrigerated: 14 days; Frozen: 30 days
All other container types
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days (whole blood unacceptable)
Room temperature: 14 days; Refrigerated: 14 days; Frozen: 30 days
All other container types
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days (whole blood unacceptable)
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
Diagnosis of active herpes simplex infection.
Performing Laboratory
med fusion
