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Herpes Simplex Virus, Type 1&2, Qualitative, Real‐Time PCR : 34257
MessagePlease order NTC 90569 for HSV 1 and 2 testing from PAP vial (test code HSVPAP).
Test Code
HSVPQL or 34257
CPT Codes
87529x2
Includes
HSV 1 and HSV 2
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in Serum separator tubes (SST). Transfer serum into sterile leak‐proof container and store refrigerated or frozen.
CSF: Collect in a sterile leak‐proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label)
Bronchial wash and BAL: Collect in a sterile leak‐proof container.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in Serum separator tubes (SST). Transfer serum into sterile leak‐proof container and store refrigerated or frozen.
CSF: Collect in a sterile leak‐proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label)
Bronchial wash and BAL: Collect in a sterile leak‐proof container.
Transport Container
Preferred Specimens
1 mL (Minimum 0.3 mL) CSF, serum, pleural fluid, pericardial fluid, amniotic fluid collected in a sterile leak‐proof container.
1 mL (Minimum 0.3 mL) Whole blood collected in: EDTA (lavender‐top) tube, Plasma collected in EDTA (lavender‐top) tube or PPT tube.
Swab collected in a viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label).
0.5 mL vitreous fluid collected in a sterile leak‐proof container.
Alternative Specimens
Bronchoalveolar lavage or bronchial wash.
1 mL (Minimum 0.3 mL) CSF, serum, pleural fluid, pericardial fluid, amniotic fluid collected in a sterile leak‐proof container.
1 mL (Minimum 0.3 mL) Whole blood collected in: EDTA (lavender‐top) tube, Plasma collected in EDTA (lavender‐top) tube or PPT tube.
Swab collected in a viral transport media, VCM, M4, or APTIMA® Unisex Collection device (white label).
0.5 mL vitreous fluid collected in a sterile leak‐proof container.
Alternative Specimens
Bronchoalveolar lavage or bronchial wash.
Transport Temperature
Refrigerated.
Stored Frozen specimens: Transport Frozen.
Stored Frozen specimens: Transport Frozen.
Specimen Stability
APTIMA® Unisex Collection device (white label)
Room temperature: 14 days; Refrigerated: 14 days; Frozen: 30 days
All other container types
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days (whole blood unacceptable)
Room temperature: 14 days; Refrigerated: 14 days; Frozen: 30 days
All other container types
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days (whole blood unacceptable)
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Limitations
Laboratory test results should always be considered in the context of clinical observations. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
Reference Range
Not Detected
Clinical Significance
Diagnosis of active herpes simplex infection.
Performing Laboratory
med fusion
