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Herpesvirus 6 (HHV‐6) DNA, Qualitative Real‐Time PCR : 16001
Test CodeHV6PQL or 16001
CPT Codes
87532
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separateplasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.
Bronchoalveolar Lavage and amniotic fluid: Collect in a sterile, plastic container and store refrigerated or frozen (Preferred).
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Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separateplasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.
Bronchoalveolar Lavage and amniotic fluid: Collect in a sterile, plastic container and store refrigerated or frozen (Preferred).
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Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
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1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) amniotic fluid collected in a sterile, leak‐proof container.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) amniotic fluid collected in a sterile, leak‐proof container.
Transport Temperature
Refrigerated
Stored Frozen specimens: Transport Frozen.
Stored Frozen specimens: Transport Frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Limitations
This test was developed and its analytical performance
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by FDA. This assay has
been validated pursuant to the CLIA regulations and is
used for clinical purposes.
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by FDA. This assay has
been validated pursuant to the CLIA regulations and is
used for clinical purposes.
Reference Range
Not Detected
Clinical Significance
This test is used to determine the presence of HHV‐6 DNA in patients' specimens. Organisms may be detected by PCR prior to detection by immunological methods. PCR provides more rapid results than other methods, including culture.
Performing Laboratory
med fusion
