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Herpesvirus 6 (HHV‐6) DNA, Quantitative, Real‐Time PCR : 19723
Test CodeHV6PQT or 19723
CPT Codes
87533
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifugewithin 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST ®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen
.
Bronchoalveolar Lavage and amniotic fluid: Collect in a sterile, plastic container and store refrigerated or frozen (Preferred).
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifugewithin 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST ®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen
.
Bronchoalveolar Lavage and amniotic fluid: Collect in a sterile, plastic container and store refrigerated or frozen (Preferred).
Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) amniotic fluid collected in a sterile, leak‐proof container.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) amniotic fluid collected in a sterile, leak‐proof container.
Transport Temperature
Refrigerated.
Stored Frozen specimens: Transport Frozen.
Stored Frozen specimens: Transport Frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
This test is used to determine the presence of HHV‐6 DNA in patients' specimens. Organisms may be detected by PCR prior to detection by immunological methods. PCR provides more rapid results than other methods, including culture.
Reportable range is 500 to 2,000,000 copies/mL (2.70 to 6.30 log copies/mL).
Reportable range is 500 to 2,000,000 copies/mL (2.70 to 6.30 log copies/mL).
Performing Laboratory
med fusion
