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Epstein Barr Virus DNA Quantitative Real‐Time PCR : 12778
Test CodeEBVPQN or 12778
CPT Codes
87799
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.
Bronchoalveolar Lavage: Collect in a sterile leakproof container.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; Serum Separator Tubes (SST®s) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, plastic, screw‐cap aliquot tubes. Freeze and ship frozen.
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.
Bronchoalveolar Lavage: Collect in a sterile leakproof container.
Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube(SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
.
Alternative Specimen
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube(SST®).
1 mL (Minimum 0.5 mL) CSF collected in a sterile, plastic, leak‐proof container.
.
Alternative Specimen
1 mL (Minimum 0.5 mL) Bronchoalveolar lavage collected in a sterile, plastic, leak‐proof container.
Transport Temperature
Refrigerated.
Stored Frozen specimens: Transport Frozen.
Stored Frozen specimens: Transport Frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
Monitoring EBV DNA levels by quantitative PCR in patients at risk of EBV‐associated lymphoproliferative disorders may allow timely recognition of virus reactivation and permit installment of antiviral treatment.
This is a quantitative molecular test, with a linear range of 200 to 2,000,000 IU/mL (2.30‐6.30 Log IU/mL).
This is a quantitative molecular test, with a linear range of 200 to 2,000,000 IU/mL (2.30‐6.30 Log IU/mL).
Performing Laboratory
med fusion
