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Epstein Barr Virus DNA Qualitative Real‐Time PCR : 34179
Test CodeEBVQL or 34179
CPT Codes
87798
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Ocular fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Ocular fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF, Lower respiratory specimens (BAL, bronchial wash, tracheal secretions), or Body fluids (amniotic fluid, ocular fluid, Minimum 0.3 mL) collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF, Lower respiratory specimens (BAL, bronchial wash, tracheal secretions), or Body fluids (amniotic fluid, ocular fluid, Minimum 0.3 mL) collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
Transport Temperature
Refrigerated.
Tissue and stored Frozen specimens: Transport Frozen.
Tissue and stored Frozen specimens: Transport Frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Limitations
This test was developed and its analytical performance
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by FDA. This assay has
been validated pursuant to the CLIA regulations and is
used for clinical purposes.
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by FDA. This assay has
been validated pursuant to the CLIA regulations and is
used for clinical purposes.
Reference Range
Not Detected
Clinical Significance
Infection with EBV is common and is generally subclinical or presents as a self‐limited illness.Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.
Performing Laboratory
med fusion
