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Epstein Barr Virus DNA Qualitative Real‐Time PCR : 34179
Test CodeEBVQL or 34179
CPT Codes
87798
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Ocular fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screwcapped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Ocular fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF, Lower respiratory specimens (BAL, bronchial wash, tracheal secretions), or Body fluids (amniotic fluid, ocular fluid, Minimum 0.3 mL) collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube (SST®).
1 mL (Minimum 0.5 mL) CSF, Lower respiratory specimens (BAL, bronchial wash, tracheal secretions), or Body fluids (amniotic fluid, ocular fluid, Minimum 0.3 mL) collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
Transport Temperature
Refrigerated.
Tissue and stored Frozen specimens: Transport Frozen.
Tissue and stored Frozen specimens: Transport Frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real‐Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
Infection with EBV is common and is generally subclinical or presents as a self‐limited illness.Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.
Performing Laboratory
med fusion
