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ANA SCREEN BY IFA WITH REFLEX TO TITER/PATTERN AND SEROLOGY CASCADE, Serum
MessagePlease see the test change notification for additional information
Test Code
LAB1231037
CPT Codes
86038, 83516
Includes
ANA by IFA. Positive ANA screens will reflex to possibly trigger AB results for dsDNA, Chromatin, Sm, SM/RNP, RNP, Jo-1, SCL 70, SSA, SSB, Centromere, RibosomeANA by IFA. Positive ANA screens will reflex to possibly trigger AB results for dsDNA, Chromatin, Sm, SM/RNP, RNP, Jo-1, SCL 70, SSA, SSB, Centromere, Ribosome
Preferred Specimen
Gold top tube 5 mL
Pediatric volume: 3 Gold top tube microtainers
Pediatric volume: 3 Gold top tube microtainers
Minimum Volume
1 mL serum
Transport Temperature
Refrigerator
Specimen Stability
Refrigerated 72 hours Frozen: Not specified
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Failure to fully fill tube
Methodology
IFA / Multiplex Immunoassay
Setup Schedule
M-F, day shift
Reference Range
Negative for all testing
Clinical Significance
Cascading starts with the most likely clinical disease associations and then moves to the least likely, in three tiers of assessment.:
Tier 1 – dsDNA, chromatin, Sm, Sm/RNP, RNP (Systemic Lupus Erythematosus, SLE/and Mixed Connective Tissue Disease/MCTD)
Tier 2 – SS-A, SS-B, Scl-70, Jo-1 (Sjogren Syndrome, Systemic Sclerosis, and Polymyositis)
Tier – 3 Centromere B, Ribosomal P (CREST syndrome and Neuropsychiatric SLE)
Serological antibody cascade used Bio-Rad BioPlex 2200 multiplex technology. Serology testing is not in itself diagnostic; connective tissue disease diagnosis requires clinical evaluation as well. If the ANA IFA test is positive but the included antibody tests are not detected, correlate with clinical findings to guide further investigation.
If overlap syndrome is being considered, separately-orderable versions of all of the serology tests are available for clinical use. See the ICAP (International Consensus on ANA Patterns) website for more information on ANA patterns, antibody associations, and research baking the classification approach:anapatterns.org
Tier 1 – dsDNA, chromatin, Sm, Sm/RNP, RNP (Systemic Lupus Erythematosus, SLE/and Mixed Connective Tissue Disease/MCTD)
Tier 2 – SS-A, SS-B, Scl-70, Jo-1 (Sjogren Syndrome, Systemic Sclerosis, and Polymyositis)
Tier – 3 Centromere B, Ribosomal P (CREST syndrome and Neuropsychiatric SLE)
Serological antibody cascade used Bio-Rad BioPlex 2200 multiplex technology. Serology testing is not in itself diagnostic; connective tissue disease diagnosis requires clinical evaluation as well. If the ANA IFA test is positive but the included antibody tests are not detected, correlate with clinical findings to guide further investigation.
If overlap syndrome is being considered, separately-orderable versions of all of the serology tests are available for clinical use. See the ICAP (International Consensus on ANA Patterns) website for more information on ANA patterns, antibody associations, and research baking the classification approach:anapatterns.org
Performing Laboratory
West Virginia University Hospitals, Inc.
Additional Information
Test Change Notification
