MDM2 (12q15) Amplification, Well-Differentiated Liposarcoma/Atypical Lipomatous Tumor, FISH, Tissue

Message

Must have H&E marked by pathologist indicating area of tumor

Test Code

LAB1230877

Alias/See Also

Lipoma
WDL/ALT
Osteosarcoma

CPT Codes
88271x2, 88274x1, 88291 x1

Includes

FISH analysis using probes for MDM2 gene and Chromosome 12 centromere and an interpretive report

Preferred Specimen

Formalin-fixed paraffin-embedded tissue slides

Minimum Volume

Two 3-micron unstained recuts and one marked H&E slide

Instructions

Please submit 2 consecutive unstained, 3-micron-thick sections placed on positively charged slides, and 1 matching hematoxylin and eosin-stained slide marked by pathologist for tumor area.

Transport Temperature

Ambient

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Will not reject, however decalcified tissues or tissues processed with unacceptable fixative may not yield a result

Methodology
FISH (fluorescence in situ hybridization)

Setup Schedule

Monday - Friday

Reference Range

Average MDM2:Average CEP12 ≤2.0 are considered negative for MDM2 amplification.

Clinical Significance

Supporting a diagnosis of well-differentiated liposarcoma/atypical lipomatous tumor Supporting a diagnosis of low-grade central or parosteal osteosarcoma

Performing Laboratory
West Virginia University Hospital, Inc.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.