BORDETELLA PERTUSSIS ANTIBODIES, IGA AND IGG BY ELISA WITH REFLEX TO IMMUNOBLOT

Message

CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR and/or Bordetella pertussis Culture. However, if serology is used to assess late-stage pertussis (>4 weeks), this is the recommended test.

If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.

Test Code

LAB1234168

CPT Codes
86615 x2; if reflexed, add 86615 for each Immunoblot

Preferred Specimen

Gold top serum 1.0 mL

Minimum Volume

0.3mL Gold Top Serum

Instructions

Specimen Preparation:Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Transport Container

ARUP Standard Transport Tube

Transport Temperature

Refrigerated

Specimen Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
New York State Clients: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.

Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Setup Schedule

Tuesday and Friday

Report Available

1-5 days

Reference Range

Bordetella pertussis Antibody, IgA by ELISA
0.9 IV or less: Negative - No significant level of detectable Bordetella pertussis IgA antibody.

1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.

1.2 IV or greater: Positive - IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.

Bordetella pertussis Antibody IgG by ELISA
0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.

0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.

1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Performing Laboratory
ARUP Laboratories

Additional Information
ARUP Laboratories

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.