KRAS MUTATION ANALYSIS, TUMOR WITH EXTENDED RAS/RAF REFLEX

Message

If the sample is negative for a KRAS mutation (GLY12ALA, GLY12ASP, GLY12ARG, GLY12CYS, GLY12SER, GLY12VAL, GLY13ASP), then reflex testing for extended RAS/RAS will be performed at additional cost.

Test Code

CPT Codes
81275 - KRAS gene analysis, 88381 - Microdissection

Includes

Identifiying colorectalconcers (CRC) without KRAS mutations thay may respond to tyrosinekinase inhibitor (TKI) therapies.

Preferred Specimen

Tumor Tissue from ColorectalCancer
Tumor tissue embedded in a formalin-fixed paraffin-embedded(FFPE) block
Core biopsy: 10 unstained, 5 micron thick sections,nonbaked
Larger tissue sections: 3 unstained, 5 micron thicksections, nonbaked

Other Acceptable Specimens

Lung cancer (FFPE)
Other cancer (FFPE)

Instructions

UML Lab Processing Instructions: Transport specimens to the Molecular Diagnostics Laboratory
WVUH Lab Processing Instructions: Slides will be brought to the Main Laboratory by cytology and placed in the ambient bin for Molecular Diganostics.

Transport Temperature

Ship with ice pack during summer months.

Specimen Stability

FFPE Block: No expiration, stored at room temperature.
Nonbaked slides: 4 weeks prior to DNA extraction, stored atroom temperature
Stained slides CANNOT be used for DNA extraction.

Methodology
Real Time PCR. The following mutations can be detected using the Qiagen therascreen KRAS RCQ PCR Kit: GLY 12 ALA, GLY 12 ASP, GLY 12 ARg, GLY 12 CYS, GLY 12 SER, GLY 12 VAL, GLY 13 ASP

Reference Range

Wild Type (No Mutation Detected). An interpretive report will be provided.

Performing Laboratory
West Virginia University Hospital Inc.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.