KRAS MUTATION ANALYSIS, TUMOR

Test Code

LAB12308001

CPT Codes
81275 KRAS gene analysis, 88381 Microdissection

Includes

Identifiying colorectal concers (CRC) without KRAS mutations thay may respond to tyrosine kinase inhibitor (TKI) therapies.

Preferred Specimen

Tumor Tissue from ColorectalCancer:
Tumor tissue embedded in a formalin-fixed paraffin-embedded(FFPE) block
Core biopsy: 10 unstained, 5 micron thick sections,nonbaked
Larger tissue sections: 3 unstained, 5 micron thicksections, nonbaked

Other Acceptable Specimens

Lung cancer (FFPE)
Other cancer (FFPE)

Instructions

UML: Ship with ice pack during summer months. Transport specimens to the Molecular Diagnostics Laboratory
WVUH Processing Lab: Slides will be brought to the Main Laboratory by cytology and placed in the ambient bin for Molecular Diagnostics.

Transport Temperature

Ship with icde pack during summer months.

Specimen Stability

FFPE Block: No expiration, stored at room temperature.
Nonbaked slides: 4 weeks prior to DNA extraction, stored at room temperature
Stained slides CANNOT be used for DNA extraction.

Methodology
Real Time PCR. The following mutations can be detected using the Qiagen therascreen KRAS RCQ PCR Kit: GLY 12 ALA, GLY 12 ASP, GLY 12 ARg, GLY 12 CYS, GLY 12 SER, GLY 12 VAL, GLY 13 ASP

Reference Range

Wild Type (No Mutation Detected). An interpretive report will be provided.

Performing Laboratory
West Virginia University Hospital, Inc.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.