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HEPARIN LOW MOLECULAR WEIGHT ANTI-XA, PLASMA
Test CodeLAB316
CPT Codes
85520
Preferred Specimen
One 3 mL blue top tube
Pediatric Volume: One 1.8 mL pediatric blue top tube.
Pediatric Volume: One 1.8 mL pediatric blue top tube.
Minimum Volume
Required: 1 mL plasma
Instructions
Sample should be collected 4-6 hours after subcutaenous injection. Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000rpm for 10 minutes or using a high speed centrifuge. Platelet poor plasma should be aliquoted into 1mL vials.
Courrier Samples: Freeze within one hour of collection. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000rpm for 10 minutes or using a high speed centrifuge. Platelet poor plasma should be aliquoted into 1mL vials.
Courrier Samples: Freeze within one hour of collection. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (two 1 mL plasma aliquots)
Specimen Stability
Ambient: Unstable Frozen: Freeze plasma in 2 aliquots of 1mL each
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted sample, underfilled tube, or gross hemolysis
Methodology
Chromogenic anti-Xa assay on ACL TOP
Setup Schedule
Sunday - Saturday Note: This test is now available 24/7 and does not require a call to the Hematology lab.
Clinical Significance
LMWH: Therapeutic range is 0.50-1.00 IU/mL for twice daily dosing and 1.00-2.00 U/mL for once daily dosing when the sample is obtained
4-6 hours after subcutaneous injection.
4-6 hours after subcutaneous injection.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center Garrett Regional Medical Center
