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VITAMIN D 25, TOTAL
MessageThis is the primary test used to identify vitamin D insufficiency.
Test Code
LAB123264
CPT Codes
82306
Preferred Specimen
Adult: One Gold Top Tube
Pediatric: 2 Gold Top Microtainers
Pediatric: 2 Gold Top Microtainers
Minimum Volume
0.5mL Serum
Other Acceptable Specimens
One Red TopTube
Instructions
Centrifuge within 1 hour of collection. Separate serum from cells if Red Top Tupe. Aliquot 0.5mL serum
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 7 days
Frozen: 3 months
Frozen: 3 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Moderate or Gross Hemolysis, Gross Lipemia
Methodology
Competitive Immunoassay
Reference Range
30-100 ng/mL
Per Endocrine Society:
Deficient if ≤ 20 ng/mL
Insufficient if 21-29 ng/mL
Sufficient if ≥ 30 ng/mL
Per Endocrine Society:
Deficient if ≤ 20 ng/mL
Insufficient if 21-29 ng/mL
Sufficient if ≥ 30 ng/mL
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center Barnesville Hospital Braxton County Memorial Camden Clark Medical Center Garrett Regional Medical Center Jackson General Hospital Jefferson Medical Center Princeton Community Hospital Potomac Valley Hospital Reynolds Memorial Hospital Summersville Regional Medical Center St. Joseph’s Hospital United Hospital Center Uniontown Hospital Wetzel County Hospital Wheeling Hospital