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HERPES SIMPLEX VIRUS (HSV1/ HSV2), PCR, CSF OR SWAB
Test CodeLAB1792
CPT Codes
87529
Preferred Specimen
CSF in a sterile vial or BD Culturette Swab placed in Remel M4RT viral transport media or BD UVT Universal Transport Media with flocked swab.
Minimum Volume
CSF: 0.6 mL in a sterile vial
Swab: placed in 3 mL of Remel M4RT viral transpor tmedia of flocked swab placed in 3mL of BD UVT media.
Swab: placed in 3 mL of Remel M4RT viral transpor tmedia of flocked swab placed in 3mL of BD UVT media.
Instructions
For swab collection' a BD Culturette sawb (available from Central Supply Lawson #19655) must be placed in
Remel M4RT viral transport or flocked swab placed in BD UVT media (Lawson #100163).
For CSF, follow CSF collection protocol.M4RT media with BD Culturettes is acceptable for UML Clients only.
CSF: Aliquot minimum of 0.6 mL of CSF sample into an aliquot tube. Freeze at -20°C.
Swab: Place culturette swab in Remel M4RT viral transport media. Freeze at -20°C.
Remel M4RT viral transport or flocked swab placed in BD UVT media (Lawson #100163).
For CSF, follow CSF collection protocol.M4RT media with BD Culturettes is acceptable for UML Clients only.
CSF: Aliquot minimum of 0.6 mL of CSF sample into an aliquot tube. Freeze at -20°C.
Swab: Place culturette swab in Remel M4RT viral transport media. Freeze at -20°C.
Transport Container
CSF: Aliquot tube, frozen
Swab: 3mL BD UVT, frozen
Swab: 3mL BD UVT, frozen
Transport Temperature
Frozen
Specimen Stability
Refrigerated(2-8°C): 7 days
Frozen: 4 days
Frozen: 4 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Non-validated sample types will be sent to a Referene Laboratory. Culturette swab placed into a media other than Remel M4RT Media or BD UVT Media. Calcium alginate swabs will be rejected.
Methodology
Performed by PCR using the Simplexa HSV 1 and 2 Direct assay and 3M Integrated Cyclers using an FDA cleared assay
Setup Schedule
Sunday-Saturday
Reference Range
Negative for HSV1 and HSV2 DNA
Clinical Significance
Test results must be interpreted in conjunction with clinical history, epidemiological data and other laboratory findings. Negative results do
not exclude HSV infections of the CNS and should not be used as the sole basis for treatment or patient management decisions. For
encephalitis patients with a negative HSV PCR result who have a syndrome compatible with HSV infection, consider repeating this test in
3-7 days. Infections caused by other viral or bacterial pathogens are not excluded by this test.
not exclude HSV infections of the CNS and should not be used as the sole basis for treatment or patient management decisions. For
encephalitis patients with a negative HSV PCR result who have a syndrome compatible with HSV infection, consider repeating this test in
3-7 days. Infections caused by other viral or bacterial pathogens are not excluded by this test.
Performing Laboratory
West Virginia University Hospitals, Inc.
