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INSULIN, SERUM OR PLASMA
Test CodeLAB304529
CPT Codes
83525
Preferred Specimen
7 mL gold or light green top tube
Pediatric Volume: Two gold top microtainers
Pediatric Volume: Two gold top microtainers
Minimum Volume
1 mL Blood
Other Acceptable Specimens
Red Top Tube
Instructions
Samples are usually collected after FASTING
Sample must be centrifuged within 2 hours of collection. Serum/plasma must be removed from the red cells and put into aliquot tubes following centrifugation. Aliquot 0.5 mL of serum.
Sample must be centrifuged within 2 hours of collection. Serum/plasma must be removed from the red cells and put into aliquot tubes following centrifugation. Aliquot 0.5 mL of serum.
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 2-8 °C 10 days
Frozen: -20°C 1 month
Frozen: -20°C 1 month
Methodology
Electrochemiluminescence Immunometric Assay ECLIA
Setup Schedule
Monday-Friday
Reference Range
3.2-32.1 ulU/mL
Clinical Significance
Test is used to diagnose insulinoma in conjunction with proinsulin and C-peptide measurements. During prolonged fasting, a reduced blood
glucose in conjunction with elevated insulin, proinsulin, and C-peptide suggest insulinoma.
Test is also used to manage diabetes mellitus (DM). Normally, insulin levels parallel blood glucose levels. Insulin levels generally decline in
type 1 DM. In early type 2 DM, insulin levels are either normal or elevated. In later stage type 2 DM, insulin levels decline.
Patients on insulin therapy may develop anti-insulin antibodies that may interfere with this assay.
glucose in conjunction with elevated insulin, proinsulin, and C-peptide suggest insulinoma.
Test is also used to manage diabetes mellitus (DM). Normally, insulin levels parallel blood glucose levels. Insulin levels generally decline in
type 1 DM. In early type 2 DM, insulin levels are either normal or elevated. In later stage type 2 DM, insulin levels decline.
Patients on insulin therapy may develop anti-insulin antibodies that may interfere with this assay.
Performing Laboratory
West Virginia University Hospital, Inc.
Camden Clark Medical Center
