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COOMBS, DIRECT (DAT)
Test CodeLAB274
CPT Codes
86880
Preferred Specimen
One 6 mL lavender top tube
Pediatric: One lavender top microtainer
Pediatric: One lavender top microtainer
Minimum Volume
3 mL blood (Adult)
Instructions
Please refer to Transfusion Practice Policy for further information on transfusion practices.
Patient’s red cells are tested using polyspecific antihuman globulin antisera (anti-IgG and C3). If positive, then test will be performed using monospecific reagents specific for IgG and C3. An elution and antibody identification will be performed on positive DAT specimens.
Received in Sunquest by Blood Bank only.
Patient’s red cells are tested using polyspecific antihuman globulin antisera (anti-IgG and C3). If positive, then test will be performed using monospecific reagents specific for IgG and C3. An elution and antibody identification will be performed on positive DAT specimens.
Received in Sunquest by Blood Bank only.
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 24 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Mislabeled specimen
Methodology
Hemagglutination/Hemolysis
Setup Schedule
Sunday-Saturday
Clinical Significance
This test is used to identify in-vivo coating of RBCs with IgG and/or the complement component C3d which can occur in conditions
including autoimmune hemolytic anemia, hemolytic transfusion reactions, and drug-induced hemolytic anemia.
Interpretation:
Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.
Positive: IgG or complement (C3d) is present on the surface of the red cell.
Cautions:
- Autoimmune hemolytic anemia can occur with a negative direct Coombs test.
- The direct Coombs test can be positive in some apparently healthy individuals.
including autoimmune hemolytic anemia, hemolytic transfusion reactions, and drug-induced hemolytic anemia.
Interpretation:
Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.
Positive: IgG or complement (C3d) is present on the surface of the red cell.
Cautions:
- Autoimmune hemolytic anemia can occur with a negative direct Coombs test.
- The direct Coombs test can be positive in some apparently healthy individuals.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center Braxton County Memorial Camden Clark Medical Center Garrett Regional Medical Center Jefferson Medical Center Princeton Community Hospital Potomac Valley Hospital Reynolds Memorial Hospital Summersville Regional Medical Center St. Joseph’s Hospital United Hospital Center Uniontown Hospital Wetzel County Hospital
