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PT INHIBITOR SCREEN (PT MIXING STUDY)
Test CodeLAB321
CPT Codes
85611 LOiNC: 5902-2
Preferred Specimen
Two 3 mL blue top tubes
Pediatric: Two 1.8 mL pediatric blue top tubes
Pediatric: Two 1.8 mL pediatric blue top tubes
Minimum Volume
Two 1 mL aliquots of plasma
Instructions
Tubes must be filled to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (two 1 mL plasma aliquots)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis.
Methodology
Prothrombin clot-based assay on ACL-TOP. This test includes an interpretative comment.
Setup Schedule
Monday-Friday
Reference Range
Refer to test PT for current normal range information
Performing Laboratory
West Virginia University Hospital, Inc.
United Hospital Center
