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PTT INHIBITOR SCREEN (PTT MIXING STUDY)
Test CodeLAB326
CPT Codes
85732
Preferred Specimen
Three 3 mL blue top tubes
Pediatric: Three 1.8 mL pediatric blue top tubes
Pediatric: Three 1.8 mL pediatric blue top tubes
Minimum Volume
Two 1mL aliquots of plasma
Instructions
Tubes must be filled to the etched line to perform analysis.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (four 1 mL plasma aliquots)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis.
Methodology
Activated partial thromboplastin clot-based assay. This test includes an interpretive comment. A thrombin time may be performed to exclude heparin contamination of the sample.
Setup Schedule
Monday-Friday
Reference Range
Refer to test aPTT for current normal range information.
Performing Laboratory
West Virginia University Hospital, Inc.
United Hospital Center
