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AFB CULTURE WITH STAIN (MYCOBACTERIUM CULTURE)
Test CodeLAB304905
CPT Codes
87116, 87206, 87015
Preferred Specimen
Respiratory, tissue, fluid or aspirate samples in sterile leak-proof container. Swab acceptable (non-OR locations) if dedicated for AFB culture.
Minimum Volume
1mL Respiratory,1mL Fluids, no minimum requirement for Tissue. Swabs: Suboptimal.
Not accepted for OR locations (please collect fluid or tissue). For sites not amenable to biopsy or aspiration, a single dedicated swab may be submitted. (i.e. multiple cultures are NOT performed on a single swab).
Not accepted for OR locations (please collect fluid or tissue). For sites not amenable to biopsy or aspiration, a single dedicated swab may be submitted. (i.e. multiple cultures are NOT performed on a single swab).
Instructions
Do not send specimens with attached tubing
1. Collect 3 first-morning sputum specimens for culture and smear for patients with clinical findings compatible with tuberculosis ( 3
separate days).
2. Transport specimens to the Micro lab immediately, ideally within 30 minutes of collection.
3. Specimens submitted on swabs (non-OR locations) must be designated for AFB culture. (i.e., multiple cultures are NOT
performed on a single swab)
4. AFB Recovery from E-Swabs has not been validated by WVUH Microbiology Lab.
Note: For AFB Blood Cultures, please see AFFB (Adult) or PFFB (Pediatric)
1. Collect 3 first-morning sputum specimens for culture and smear for patients with clinical findings compatible with tuberculosis ( 3
separate days).
2. Transport specimens to the Micro lab immediately, ideally within 30 minutes of collection.
3. Specimens submitted on swabs (non-OR locations) must be designated for AFB culture. (i.e., multiple cultures are NOT
performed on a single swab)
4. AFB Recovery from E-Swabs has not been validated by WVUH Microbiology Lab.
Note: For AFB Blood Cultures, please see AFFB (Adult) or PFFB (Pediatric)
Transport Temperature
Refrigerated
Specimen Stability
Refrigerated: 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Intra-operative specimen submitted on swab. Twenty-four hour specimens are not acceptable for culture. Stool cultures for AFB will only be performed on HIV positive patients. Urine in a grey top tube.
Methodology
Culture
Setup Schedule
Sunday-Saturday
Reference Range
No acid fast bacilli seen on direct smear. No growth in 6
weeks.
weeks.
Performing Laboratory
West Virginia University Hospital, Inc.
Camden Clark Medical Center
