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HEPARIN DEPENDENT ANTIBODY WITH SEROTONIN RELEASE REFLEX
MessageThis is the primary test used to diagnose heparin-induced thrombocytopenia (HIT) in conjunction with clinical parameters. A
serotonin release assay will be performed when resutls of this test do not correlate with clinical impression.
serotonin release assay will be performed when resutls of this test do not correlate with clinical impression.
Test Code
LAB304813
CPT Codes
86022
Preferred Specimen
One 3 mL BLUE top tube
Minimum Volume
1 mL Plasma
Instructions
Do not collect through heparinized line. Must be collected by venipuncture. Blue top tube must be filled properly to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (two 1 mL plasma aliquots)
Specimen Stability
Ambient: 4 hours
Frozen: 6 months (-70°C), freeze within 1 hour of collection.
Frozen: 6 months (-70°C), freeze within 1 hour of collection.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tube contaminated with heparin, gross hemolysis, specimen clotted or not filled properly.
Methodology
Latex-enhanced immunoturbidimetric method performed on ACL TOP analyzers.
Setup Schedule
Monday thru Friday
Reference Range
Negative
Clinical Significance
Pathology will discuss positive results with provider. A recommendation for SRA testing will be detereminde at that time. SRA
testing, if indicated will be ordered by Pathology. Sample collection will be performed by provider.
testing, if indicated will be ordered by Pathology. Sample collection will be performed by provider.
Performing Laboratory
West Virginia University Hospital, Inc.
