| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
BONE MARROW, ADULT AND PEDIATRIC
Test CodeLAB30449
CPT Codes
85007
Includes
Preparing cover slipped specimens at the bedside, peripheral smear description, marrow aspirate morphology, marrow biopsy, marrow differential, ME ratio, and interpretation. Other tests incluing reticulocyte count,immunohistochemistry, flow cytometry, FISH, cytogenetics, and molecular testing will be performed as indicated based on Pathologist review.
Preferred Specimen
One lavender top tube of bone marrow aspirate
Minimum Volume
1 cc bone marrow aspirate and an additional 1 cc for each additional test needed
Instructions
Assisted bone marrow collection must be scheduled in advance with the Hematology Lab at 74230.
Transport to lab should be the same day as collection. Test should be scheduled with the Hematology Lab. Bone marrow aspirate and slides should not be placed in the same bag as a formalin sample due to cross reactivity of formalin and the staining process.
Samples from Outside Locations: The bone marrow aspirate and slides cannot be placed in the same bag as a formalin specimen due to
the cross reactivity with the staining process.
Transport to lab should be the same day as collection. Test should be scheduled with the Hematology Lab. Bone marrow aspirate and slides should not be placed in the same bag as a formalin sample due to cross reactivity of formalin and the staining process.
Samples from Outside Locations: The bone marrow aspirate and slides cannot be placed in the same bag as a formalin specimen due to
the cross reactivity with the staining process.
Transport Temperature
Refrigerate: transport time > 4 hours
Specimen Stability
Unstable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Too few cells forevaluation.
Any slides submitted for testing or review must be labeled with two patient identifiers. Acceptable identifiers include Name, MRN, Birth date, Accession/SID.
Any slides submitted for testing or review must be labeled with two patient identifiers. Acceptable identifiers include Name, MRN, Birth date, Accession/SID.
Methodology
Manual
Setup Schedule
Monday-Friday
Reference Range
Adults:
Pediatrics:
| Cell Differential | Reference Values (%) |
| Blasts | 0-3 |
| Promyelocytes | 1-5 |
| Myelocytes | 6-16 |
| Metamyelocytes | 4-15 |
| Segmented neutrophils and bands | 15-38 |
| Monocytes | 0-4 |
| Eosinophils | 0-6 |
| Basophils | 0-1 |
| Erythroid Precursors | 17-39 |
| Lymphocytes | 8-22 |
| Plasma Cells | 0-4 |
| M:E Ratio | 2-4:1 |
Pediatrics:
| Cell Differential | Birth | 1 Week | 1 Week- 1 year | 1-4 years | 4-12 years |
| Blasts | 0-3 | 0-3 | 0-3 | 0-3 | 0-3 |
| Granulocytic Series | 31-77 | 21-79 | 22-52 | 32-68 | 35-69 |
| Monocytes | 0-2 | 0-2 | 0-2 | 0-4 | 0-4 |
| Eosinophils | 0-5 | 0-5 | 0-2 | 2-10 | 2-10 |
| Basophils | 0-1 | 0-1 | 0-1 | 0-1 | 0-1 |
| Erythroid Precursors | 18-50 | 10-25 | 7-27 | 11-27 | 11-31 |
| Lymphocytes | 2-10 | 7-19 | 15-54 | 8-36 | 12-28 |
| Plasma Cells | <1 | <1 | <1 | 0-2 | 0-2 |
| M:E Ration (mean) | 1.6:1 | 4.3:1 | 2.2:1 | 2.6:1 | 2.5:1 |
Performing Laboratory
West Virginia University Hospital, Inc.
