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PTT (ACTIVATED PARTIAL THROMBOPLASTIN TIME)
Test CodeLAB325
CPT Codes
85730
Preferred Specimen
One 3 mL blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Minimum Volume
1 mL plasma
Instructions
Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (1 mL plasma aliquot)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis.
Methodology
Optical Density on ACL TOP using SynthASil aPTT reagent
Setup Schedule
Sunday-Saturday
Reference Range
24.2 - 37.5 sec
Note: The upper reference range limit may be higher in newborns (e.g. 40-45 sec)
Automatically Called Results > 120.0 sec
No Result: Analyzer Fails to obtain results. No Clotting deteced during testing. Please repeat if clinically indicated.
Note: The upper reference range limit may be higher in newborns (e.g. 40-45 sec)
Automatically Called Results > 120.0 sec
No Result: Analyzer Fails to obtain results. No Clotting deteced during testing. Please repeat if clinically indicated.
Clinical Significance
Interpretive Information
Therapeutic range for unfractionated heparin is 60.0 to 100.0 seconds.
Direct oral anticoagulants can effect coagulation testing results.
Therapeutic range for unfractionated heparin is 60.0 to 100.0 seconds.
Direct oral anticoagulants can effect coagulation testing results.
Performing Laboratory
West Virginia University Hospital, Inc.
Bluefield Pavilion Campus Berkeley Medical Center Barnesville Hospital Braxton County Memorial Camden Clark Medical Center Camden Clark Medical Center Fairmont Medical Center Garrett Regional Medical Center Harrison Community Hospital Jackson General Hospital Jefferson Medical Center Princeton Community Hospital Potomac Valley Hospital Reynolds Memorial Hospital Summersville Regional Medical Center St. Joseph’s Hospital United Hospital Center Uniontown Hospital Wetzel County Hospital Wheeling Hospital
