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HEPATITIS B SURFACE ANTIBODY
Test CodeLAB472
CPT Codes
86706
Preferred Specimen
One 5 mL gold top tube
Minimum Volume
2 mL blood
Required: 1.0 mL serum
Required: 1.0 mL serum
Other Acceptable Specimens
Red top, light green or dark green top tube
Instructions
Avoid freeze-thaw cycling
Sample must be centrifuged within two hours of collection. Serum/plasma must be removed from the red cells and put into aliquot tubes following centrifugation.
Sample must be centrifuged within two hours of collection. Serum/plasma must be removed from the red cells and put into aliquot tubes following centrifugation.
Transport Temperature
Refrigerated
Specimen Stability
Centrifuge within 2 hours after collection
Ambient: 3 days Refrigerated: 7 days Frozen: not specifiedRequired: 1.0 mL serum
Ambient: 3 days Refrigerated: 7 days Frozen: not specifiedRequired: 1.0 mL serum
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Turbid, lipemic, grossly hemolyzed, or heat-inactivated specimens. Cadaveric specimens
Methodology
Chemiluminescent Microparticle Immunoassay
Setup Schedule
Sunday- Saturday
Reference Range
< 8 mIU/mL=Negative or Unvaccinated
≥8 and < 12 mIU/mL= Equivocal/Indeterminate
≥12mIU/mL- Reactive or Vaccinated
≥8 and < 12 mIU/mL= Equivocal/Indeterminate
≥12mIU/mL- Reactive or Vaccinated
Clinical Significance
A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B virus. Assay does not differentiate between vaccines and natural infections.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center Camden Clark Medical Center Reynolds Memorial Hospital St. Joseph’s Hospital United Hospital Center Uniontown Hospital Wheeling Hospital
