LUPUS ANTICOAGULANT SCREEN

Test Code

LAB319

CPT Codes
85613 (LAC), 85730 (SCT)

Preferred Specimen

Two 3 mL blue top tube
Pediatric Volume: Three 1.8 mL pediatric blue top tubes

Minimum Volume

Required for Testing: Two 1 mL aliquots of platelet-free plasma

Instructions

Tube must be filled to etched line to perform testing.

Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.

Transport Temperature

Frozen: two 1 mL platelet-free aliquots (platelet count < 5,000)

Specimen Stability

Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Clotted specimen, underfilled tube, or gross hemolysis

Methodology
Lupus Anticoagulant (LAC) Screen and LAC Confirm based on Diluted Russell's Viper Venom clotting time. Silica clotting time (SCT) Screen and SCT Confirm based on direct activation of clotting.

Setup Schedule

Tuesday and Friday

Reference Range

Negative. LAC Normalized ratio ≤1.20
SCT Normalized ratio ≤1.16

Clinical Significance

This screen for lupus anticoagulants include 2 screening tests (LAC screen/confirm & SCT screen/confirm) that include confirmatory testing
for phospholipid dependence. An interpretive comment is included in the test result.

Performing Laboratory
West Virginia University Hospital, Inc.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.