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ANTITHROMBIN ACTIVITY, PLASMA
Test CodeLAB311
CPT Codes
85300 LOINC: 27811-9
Preferred Specimen
One 3.0 mL blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Minimum Volume
1 mL Plasma
Instructions
Tube must be filled to the etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, unfilled tube,gross hemolysis or excess anticoagulants
Methodology
Chromogenic assay on ACL TOP
Setup Schedule
Sunday-Saturday Samples performed each day at 10am. ECMO patients are performed on demand.
Reference Range
83-128%
Performing Laboratory
West Virginia University Hospital, Inc.
