METHADONE CONFIRMATORY/ DEFINITIVE, URINE, BY LC-MS/MS

Message

Useful For
• Definitive compliance checks in patients prescribed methadone
• Confirming presumptive positive screening tests for methadone [Urine quantitative values do NOT correlate well with serum
concentrations and should not be used for assessing dose efficacy or timing.]

Test Code

LAB1230616

CPT Codes
80307

Includes

Quantitative/definitive detection of methadone and its primary metabolite, EDDP (2-Ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine). Quantitative results are only provided for positive results; negative results will have an interpretation of “Negative” and a component result of “Not detected”. [Urine quantitative values do NOT correlate well with serum concentrations and should not be used for assessing dose efficacy or timing.] Adulteration testing is provided at no-charge and includes urine creatinine

Preferred Specimen

Urine, random specimen clean plastic collection cup

Minimum Volume

5mL
Pediatric volume: 3mL

Instructions

Specimens collected in clean plastic collection cups are acceptable. Store refrigerated for best stability.
Transfer mixed urine specimen to a labeled red top tube and deliver to Chemistry department for track-based processing, adulteration testing,
and aliquoting for use in the mass spectrometry section.

Transport Temperature

Refrigerated

Specimen Stability

Ambient: 24 hours Refrigerated: 10 days

Methodology
LC-MS/MS

Setup Schedule

Monday - Friday Dayshift

Reference Range

Negative

Clinical Significance

Cutoffs:
EDDP, 50 ng/mL
Methadone, 50 ng/mL

Performing Laboratory
West Virignia University Hospitals, Inc

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.