SYPHILIS SCREENING ALGORITHM WITH REFLEX, SERUM

Message

Please see the link below under additional information for the test change update.

Test Code

LAB1230000

CPT Codes
The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.

Preferred Specimen

Gold Top Tube
Pediatric: 2 Gold top Microtainers

Minimum Volume

Required for Testing: 0.5mL

Other Acceptable Specimens

Red Top Tube

Instructions

Centrifuge within 1 hour of collection. Separate serum from cells if Red Top Tube. Aliquot 0.5mL serum

Transport Temperature

Refrigerated

Specimen Stability

Ambient: 72 hours
2-8°C for 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Failure to fully fill tube, Gross hemolysis

Methodology
Chemiluminescent Immunoassay

Setup Schedule

Sunday-Saturday

Reference Range

Nonreactive
Will reflex to RPR with or without titer and/or TP-PA if necessary (see Clinical Significance).

Clinical Significance

SYPHILIS SCREENING ALGORITHM with REFLEX, Serum
This algorithm starts with a treponemal antibody screening test, and results are reported as Nonreactive or Reactive. All Reactive results are automatically reflexed to RPR (rapid plasma regain) confirmation testing with endpoint titer reported for reactive samples. Supplemental TP-PA is performed if treponemal antibody screen and RPR results are discordant. All tests utilize FDA-cleared reagents verified by WVUH laboratories.

Treponemal Antibodies	RPR Result	TP-PA Result	Conclusion/Interpretation
Nonreactive	Not performed	Not performed	No serologic evidence of exposure to syphilis.
Reactive	Reactive	Not performed	Suggestive of active infection. Treponemal and non-treponemal antibodies detected.
Reactive	Nonreactive	Reactive	Suggestive of remote infection with treatment/resolution OR late stage infection. The presence of treponemal antibody is confirmed, but no evidence of non-treponemal antibody is detected.
Reactive	Nonreactive	Nonreactive	Suggestive of false positive treponemal antibody screening result. The presence of treponemal antibody is not confirmed, and there is no evidence of non-treponemal antibody detected.
Reactive	Nonreactive	Indeterminate	Indeterminate. The presence of treponemal antibody is equivocal, and there is no evidence of non-treponemal antibody detected. Recommend repeat testing in 4-6 weeks.

Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center
Camden Clark Medical Center
St. Joseph’s Hospital
United Hospital Center

Additional Information
Test change notification

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.