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HIV-1 RNA QUANTITATIVE PCR, PLASMA
Test CodeLAB304213
CPT Codes
87536
Includes
Detection of HIV virus group Mand O by real-time PCR.
Preferred Specimen
Two 6 mL lavender top tubes
Minimum Volume
5 mL blood
Instructions
Plasma must be separated from cells within 24 hours of collection.
Aliquot 2.5 mL of plasma and place aliquot in the -20 freezer
Aliquot 2.5 mL of plasma and place aliquot in the -20 freezer
Transport Temperature
Frozen
Specimen Stability
For plasma separated from cells within 24 hours of collection
Refrigerated: 6 days Frozen: 12 weeks (preferred storage)
Refrigerated: 6 days Frozen: 12 weeks (preferred storage)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Any specimen type other than plasma. Heparinized tubes are not acceptable due to inhibition ofthe PCR reaction.
Methodology
Polymerase Chain Reaction (PCR)
FDA Status
FDA Approved
Setup Schedule
Monday - Friday (varies)
Reference Range
Target not Detected
Clinical Significance
EDTA plasma specimens are analyzed by the Roche COBAS 6800 HIV assay. This is a FDA approved test.
Performing Laboratory
West Virginia University Hospital, Inc.