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METHOTREXATE LEVEL
Test CodeLAB481
CPT Codes
80299
Preferred Specimen
One 6 mL RED top tube. Protect from light.
Pediatric: One red top microtainer
Pediatric: One red top microtainer
Other Acceptable Specimens
Dark green top tube
Instructions
Specify dosage history. Time drawn must be written on label.
Sample must be centrifuged within 2 hours of collection. Serum/plasma (red or dark green top tube) must be removed from the red cells and put into amber aliquot tubes following centrifugation.
Sample must be centrifuged within 2 hours of collection. Serum/plasma (red or dark green top tube) must be removed from the red cells and put into amber aliquot tubes following centrifugation.
Transport Temperature
Refrigerated
Specimen Stability
Unstable, centrifuge within two hours of collection and protect from light.
Ambient: 4 hours
Refrigerated: 2 weeks
Frozen: not specified
Ambient: 4 hours
Refrigerated: 2 weeks
Frozen: not specified
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples drawn in tubes containing a separator gel may cause falsely lower results.
Methodology
Enzyme Immunoassay
Setup Schedule
Sunday-Saturday
Reference Range
Reference Interval: 0.5-5.0 umol/L
Critical Value: ≥5 umol/L
Critical Value: ≥5 umol/L
Performing Laboratory
West Virginia University Hospital, Inc.
