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PROTEIN S ANTIGEN, FREE, PLASMA
MessageThis is the primary test used at WVUH to screen for protein S deficiency. It has replaced the clot-based protein S activity assay.
Test Code
LAB304620
CPT Codes
85306
Preferred Specimen
One 3 mL blue top tube
Pediatric: One 1/8 mL pediatric blue top tube
Pediatric: One 1/8 mL pediatric blue top tube
Minimum Volume
Required for Testing: 1 mL plasma
Instructions
Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Transport Temperature
Frozen (two 1 mL plasma aliquots)
Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)
Frozen: 6 months (-70C)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Reject Due to: Clotted specimen, improperlyfilled tube, or gross hemolysis.
Methodology
Latex immunoassay on the ACL TOP
Setup Schedule
Tuesday and Thursday
Reference Range
Male: 74.1-146.1%
Female: 54.7-123.7%
Female: 54.7-123.7%
Clinical Significance
Patients should be off oral anticoagulation therapy (Coumadin) for two weeks to accurately measure protein S levels
Performing Laboratory
West Virginia University Hospital, Inc.
