CD20 B CELL COUNT BY FLOW CYTOMETRY, PERIPHERAL BLOOD

Message
This is a new test effective 11/01/2017. Assessing therapeutic B-cell depletion quantitatively in any clinical context, including malignancies, autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, and membranous glomerulonephritis among others, and treatment or prevention of acute humoral rejection in positive crossmatch renal transplant recipients. Evaluation of CD19 deficiency in patients with a suspected CD19 deficiency (humoral immunodeficiency). This test should not be ordered for a comprehensive evaluation of peripheral B-cell subsets. This test should not be used for evaluating presence of CD20 on malignant or nonmalignant B cells.


Test Code
LAB123271


CPT Codes
88184, 88185

Includes
Percent CD19 and CD20 Bcells,  Absolute CD19 and CD20 B cells


Preferred Specimen
One 3 mL Lavender top tube


Minimum Volume
1 mL blood


Instructions
Deliver to Flow Lab immediately (Monday-Friday day shift). Refrigerate specimen when Flow Lab is closed


Transport Temperature
Refrigerate


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Improper tube type drawn
Frozen sample


Methodology
Flow Cytometry

Setup Schedule
Monday-Friday


Reference Range
Appropriate age-specific reference values are provided on the report.


Performing Laboratory
West Virginia University Hospital, Inc.



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.