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MYELOPROLIFERATIVE NEOPLAM (MPN) DISORDERS, FISH
Test CodeLAB1238020
CPT Codes
88271 x5, 88275 x4, 88291
Preferred Specimen
Bone Marrow: 0.5-1 mL aspirate in dark green top tube (sodium heparin)
Blood: 2-3 mL whole blood in a dark green top tube (sodium heparin)
Blood: 2-3 mL whole blood in a dark green top tube (sodium heparin)
Minimum Volume
Bone Marrow: 0.5mL aspirate (sodium heparin)
Blood: 1-2 mL whole blood in a dark green top tube (sodium heparin)
Blood: 1-2 mL whole blood in a dark green top tube (sodium heparin)
Other Acceptable Specimens
Lavender top (EDTA) acceptable for FISH only (no Karyotype ordered) samples.
Instructions
Place in cytogenetics bucket near chemistry
Transport Temperature
Ambient or refrigerated (prolonged transport conditions) NEVER FROZEN
Specimen Stability
Bone Marrow: Ambient(preferred), Refrigerated (accepted). NEVER FROZEN
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Insufficient or clotted sample, more than 4 days old when received in laboratory.
Methodology
Fluorescent In Situ Hybridization (FISH) (panel includes the following probes: PDGFRalpha, PDGFRbeta, JAK2, BCR/ABL and FGFR1)
Setup Schedule
Samples processed Monday through Saturday. Results reported Monday through Friday
Reference Range
An interpretive report will be provided
Performing Laboratory
West Virginia University Hospital, Inc.
