| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
RETICULOCYTE COUNT
Test CodeLAB296
CPT Codes
85046
Includes
Percent and absolute numbers of young red cells.
Preferred Specimen
One 3mL lavender top tube
Pediatric: One lavender top microtainer
Pediatric: One lavender top microtainer
Minimum Volume
1mL blood(Adult)
Transport Temperature
Refrigerated
Specimen Stability
Ambient: 24 hours
Refrigerated: 48 hours
Refrigerated: 48 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted or contaminated specimen
Methodology
Sysmex 9100
Setup Schedule
Sunday-Saturday
Reference Range
| Age | Retic % | Retic # (103/uL) | IRF % |
| >18Y Male | 0.50-2.20 | 26.0-110.0 | 2.7-15.9 |
| >18Y Female | 0.50-2.20 | 17.-100.0 | 2.7-15.9 |
| 12Y-18Y | 0.9-1.5 | 42.0-65.0 | 9.0-18.7 |
| 6Y-12Y | 1.0-1.9 | 42.4-70.2 | 8.9-24.1 |
| 2Y-6Y | 0.8-1.5 | 36.4-68.0 | 8.4-21.7 |
| 181D-2Y | 1.0-1.8 | 43.5-111.1 | 11.4-25.8 |
| 61D-180D | 1.6-2.7 | 48.2-88.2 | 13.4-23.3 |
| 31D-60D | 2.1-3.5 | 51.8-77.9 | 19.1-28.9 |
| 4D-30D | 1.1-2.4 | 51.3-110.4 | 14.5-24.6 |
| 0D-3D | 3.5-5.4 | 147.5-216.4 | 30.5-35.1 |
Reticulocyte hemoglobin content (RET-He, pg): 28.0-38.0 (no age ranges)
Clinical Significance
Reticulocyte hemoglobin content (RET-He) measures reticulocyte hemoglobin content and can be used to assess hemoglobin available for mature RBC production. Immature reticulocyte fraction (IRF) measures the most immature forms of reticulocytes exhibiting high nucleic acid content. Depending on the appropriate clinical context, one or both of these parameters may be used to characterize erythropoietic activity in anemic patients. Some specific scenarios include the evaluation of absolute and functional iron deficiency, response to erythropoietin stimulating agents, and the evaluation of peripheral loss/destruction or marrow suppression of red blood cell production. Anemic patients with elevated IRF and/or RET-He values suggesting that RBCs are being actively produced may not require a RBC transfusion depending on the clinical situation.
Performing Laboratory
West Virginia University Hospital, Inc.
Berkeley Medical Center Barnesville Hospital Camden Clark Medical Center Fairmont Medical Center Garrett Regional Medical Center Jackson General Hospital Jefferson Medical Center Princeton Community Hospital Potomac Valley Hospital Reynolds Memorial Hospital Summersville Regional Medical Center St. Joseph’s Hospital United Hospital Center Uniontown Hospital Wheeling Hospital
