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B-CELL LYMPHOMA, FISH (BCL2, BCL6 & MYC INCLUDED)
Test CodeLAB1238009
CPT Codes
88271 X5, 88275 X3, 88291 X1
Preferred Specimen
Bone Marrow: 0.5-1 mL aspirate in a dark green top tube (sodium heparin) or
Tissue: T 5 Slides, Touch Preps (preferred) or 5 micron unstained paraffin-embedded (FFPE) tissue recut slides along with marked H&E. (Blocks can be sent and cut by Histology Department).
Tissue: T 5 Slides, Touch Preps (preferred) or 5 micron unstained paraffin-embedded (FFPE) tissue recut slides along with marked H&E. (Blocks can be sent and cut by Histology Department).
Minimum Volume
Bone Marrow: 0.5mL aspirate in a dark green top tube (sodium heparin) or
Tissue: 4 slides Touch Preps (preferred) or 3 micron unstained paraffin-embedded tissue recut slides along with marked H&E. (Blocks can be sent and cut by Histology
Tissue: 4 slides Touch Preps (preferred) or 3 micron unstained paraffin-embedded tissue recut slides along with marked H&E. (Blocks can be sent and cut by Histology
Other Acceptable Specimens
Lavender top tube (EDTA)for FISH only specimens
Instructions
Place in cytogenetics bucket near chemistry.
Transport Temperature
Ambient or refrigerated (prolonged transport conditions)
Specimen Stability
Tissue/Bone Marrow: Ambient( preferred), Refrigerated acceptable.
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bone Marrow: insufficient or clotted sample, more than 4 days old when received in laboratory.
Tissue: Necrosis of Tissue sample.
Tissue: Necrosis of Tissue sample.
Methodology
Fluorescent In Situ Hybridization (FISH) (FISH panel includes the following probes: IGH/BCL2, BCL6, MYC, and IGH/MYC
Setup Schedule
Samples processed Monday through Saturday.
Report Available
Results reported Monday through Friday
Reference Range
An interpretive report will be provided.
Performing Laboratory
West Virginia University Hospital, Inc.
