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ACUTE MYELOID LEUKEMIA (AML), FISH
Test CodeLAB1238006
CPT Codes
882710 X12, 88275 X8, 88291 X1
Preferred Specimen
Bone Marrow: 0.5-1 mL aspirate in dark green top tube (sodium heparin) or
Blood: 2-3 mL whole blood in a dark green top tube (sodium heparin)
Blood: 2-3 mL whole blood in a dark green top tube (sodium heparin)
Minimum Volume
Bone Marrow: 0.5mL aspirate (sodium heparin) or
Blood: 1-2 mL whole blood in a dark green top tube (sodium heparin)
Blood: 1-2 mL whole blood in a dark green top tube (sodium heparin)
Other Acceptable Specimens
Lavender top (EDTA) acceptable for FISH only (no Karyotype ordered) samples
Instructions
Place in cytogenetics bucket near chemistry.
Transport Temperature
Ambient or refrigerated (prolonged transport conditions) NOT FROZEN
Specimen Stability
Bone Marrow: Ambient (preferred), Refrigerated (accepted)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Insufficient or clotted sample, more than 4 days old when received, hemolysis, improper transport conditions, frozen or centrifuged.
Methodology
Fluorescent In Situ Hybridization (FISH) FISH Panel includes the following probes: PML/RARA, RUNXI/RUNXITI, CBFB (inversion 16), KMT2A (MLL), TP53, RBI, RPNI/MECOM (inversion 3), and BCR/ABL1
Setup Schedule
Samples processed Monday through Saturday.
Report Available
Results reported Monday through Friday
Reference Range
An interpretive report will be provided
Performing Laboratory
West Virginia University Hospital, Inc.
