FACTOR 5 ACTIVITY, PLASMA

Message
This test is performed to assess functional factor 5 as seen with an increased PT or APTT (bleeding problem). For clotting disorders,
please refer to Factor V Mutation , see order code FAVL FACTOR 5 MUTATION (LEIDEN).


Test Code
LAB304


CPT Codes
85220, LOiNC:3193-0

Preferred Specimen
One 3 mL blue top tube
Pediatric: One 1.8 mL pediatric blue top tube.


Minimum Volume
1 mL Plasma


Instructions
Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge.  Platelet poor plasma should be separated into 1 ml aliquots.  Samples should be frozen within one hour (best practice), however samples stable for 4 hours.  Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
 


Transport Temperature
Frozen (two 1 mL plasma aliquots)


Specimen Stability
Ambient: 4 hours if cap has not been removed or punctured
Frozen: 6 months (-70C)


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted specimen, underfilled tube, or gross hemolysis. 


Methodology
Prothrombin clot-based assay on ACL-TOP

Setup Schedule
Monday-Friday


Reference Range
62-139%


Performing Laboratory
West Virginia University Hospital, Inc.




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.