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THROMBIN TIME (BOVINE), PLASMA
Test CodeLAB324
CPT Codes
85670
Preferred Specimen
One 3 mL blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Pediatric: One 1.8 mL pediatric blue top tube
Minimum Volume
1 mL Plasma
Instructions
Tube must be filled to etched line to perform testing.
Samples must be centrifuged at 3000 rpm for 10 minutes or using a high-speed centrifuge. Platelet poor plasma should be separated into 1 ml aliquots. Samples should be frozen within one hour (best practice), however samples stable for 4 hours. Samples must remain frozen during transport. Any samples that are received thawed are not suitable for testing.
Do not draw through arterial lines. Contact lab if there is a possibility of heparin contamination.
Specimen Stability
Ambient: 4 hours
Frozen: 6 months (-70°)
Frozen: 6 months (-70°)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Thawed specimen, Clotted specimen, underfilled tube, or gross hemolysis
Methodology
Clot-based assay measuring time of fibrinogen to fibrin conversion after adding bovine thrombin on ACL TOP
Setup Schedule
Sunday-Saturday
Reference Range
10.3-16.6 seconds
Performing Laboratory
West Virginia University Hospital, Inc.
