14-3-3 Protein, CSF (Prion Disease)

37989

LAB15000

5 mL CSF collected in a sterile screw-cap vial

2 mL

Collect CSF by lumbar puncture. Discard first 2 mL that flows from tap. Collect next 5 mL CSF, avoiding bloody tap.

Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours). The NPDPSC requests a urine sample for research purposes with all CSF samples if available.

Ship Monday through Wednesday

**Please note: Patient is also asked to submit a frozen urine sample (100-200 mL) for validation of a recently published diagnostic test on Creutzfeldt-Jakob disease; no report will be issued. Please inform patient and/or family that urine is used for research on a diagnostic test and obtain oral consent.

Urine: ship double boxed using a styrofoam container with sufficient dry ice (5 lbs/24 hours).

Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
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In addition to our Test Request Form, all senders of CSF will be required to also complete and submit our CSF Billing Requisition Form only if the NPDPSC is to bill patient directly.

Frozen

Room temperature: 24 hours
Refrigerated: 14 days
Frozen: Indefinitely

Set up: Tues, Thurs; Report available: 21 days

See Laboratory Report

In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.