FISH, High-Grade Lymphoma Panel

Test Code

91606

Alias/See Also

LAB1234109

CPT Codes
88275 (x3), 88271 (x6)

Includes

100-300 interphase cells are microscopically analyzed for each of the following probe sets: Rearrangements of MYC (8q24); Rearrangements of BCL6 (3q27); IGH/BCL2 t(14;18)

Preferred Specimen

3 mL bone marrow submitted in transport medium

Minimum Volume

1 mL bone marrow

Other Acceptable Specimens

Bone marrow collected in: Sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube or sodium heparin (tan-top) tube • 5x5mm fresh lymph node/tumor biopsy in transport medium, or formalin-fixed paraffin-embedded tissue block

Instructions

Bone Marrow, 1-3 mL in transport medium (preferred) or sodium heparin (green-top, dark/royal blue-top or tan-top) tubes are acceptable containers for this test.
Lymph node/tumor: 5X5 mm fresh tumor biopsy in a transport medium.
Paraffin Block: Formalin-fixed, paraffin-embedded tissue.

Transport media available upon request.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Monday-Sunday Evening
Report available: 7 Days

Reference Range

See Laboratory Report

Clinical Significance

High-grade B-cell lymphoma can be difficult to classify if histologic and immunophenotypic features are not entirely typical. Large B-cell lymphoma with monomorphic appearance or very high mitotic rate and Burkitt lymphoma cases that have atypical immunophenotypic or morphologic appearances present diagnostic difficulties. The FISH panel for MYC (8q24), t(14;18) (IGH-BCL2) and BCL6 (3q27) translocations, provides additional diagnostic information that may help in final classification and therapy selection in high-grade B-cell lymphomas as well as to assess for "double-hit" lymphomas and "triple-hit" lymphomas.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.