Immune Assessment B Cell Subsets, Blood

Test Code

92717

Alias/See Also

LAB15080
Lymphocyte Subset Panel

CPT Codes
86355, 86356 (x7), 86357, 86359, 86360

Includes

Quantitative Lymphocyte Subsets: T, B, and NK
Immune Assessment B Cell Subsets, B

Preferred Specimen

(TBBS) Quantitative Lymphocyte Subsets:
3 mL whole blood collected in an EDTA (lavender-top) tube, and

(IABC) B-Cell Phenotyping Screen:
Ages > 14 years: 10 mL whole blood collected in an EDTA (lavender-top) tube, or
Ages ≤ 14 years: 4 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

(TBBS) Quantitative Lymphocyte Subsets: 1 mL

(IABC) B-Cell Phenotyping Screen:
Ages > 14 years: 5 mL
Ages ≤ 14 years: 3 mL

Instructions

Two separate Whole Blood EDTA specimens are required: 1 refrigerated and 1 ambient transport temperature.

Whole blood for (TBBS) Quantitative Lymphocyte Subsets: T, B, and NK:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK. Specimen Stability: Ambient
--AND--
Whole blood for (IABC) B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood.
Specimen Stability: Refrigerated

Necessary Information:
1.) Date of draw is required.
2.) Ordering physician's name and phone number are required.

Shipping Instructions: Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw. Samples arriving on the weekend and observed holidays may be canceled. Client Direct Ship Process is required.

Transport Temperature

(TBBS) Quantitative Lymphocyte Subsets: Room temperature
(IABC) B-Cell Phenotyping Screen: Refrigerated (cold packs)

Specimen Stability

(TBBS) Quantitative Lymphocyte Subsets
Room temperature: < 52 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

(IABC) B-Cell Phenotyping Screen
Room temperature: Unacceptable
Refrigerated: < 48 hours
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Gross lipemia

Methodology
TBBS: Flow Cytometry
IABC: Fluorescent Flow Cytometry

FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Setup Schedule

Set up: Mon-Fri; Report available: 3-4 days

Reference Range

See Laboratory Report

Clinical Significance

Screening for common variable immunodeficiency (CVID) and hyper-IgM syndromes Assessing B-cell subset reconstitution after stem cell or bone marrow transplant Assessing response to B-cell-depleting immunotherapy Identifying defects in transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI) and B-cell-activating factor receptor (BAFF-R) in patients presenting with clinical symptoms and other laboratory features consistent with CVID.

Performing Laboratory
Mayo Clinic Laboratories
200 First Street SW
Rochester, MN 55905

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.