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Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative Real Time PCR
Test Code34257
Alias/See Also
LAB18024
CPT Codes
87529 (x2)
Preferred Specimen
Not Accepted: CSF or Skin/Mucosal swabs: see LAB1792 Herpes Simplex Virus (HSV1/2), PCR, CSF/Swab
Pleural fluid, amniotic fluid or pericardial fluid: 1 mL collected in a sterile leak-proof container
Whole blood: 1 mL collected in an EDTA (lavender-top) or ACD (yellow-top) tube
Bronchoalveolar lavage or bronichial wash collected in a sterile leak-proof container.
Vitreous fluid: 0.5 mL collected in a sterile leak-proof container
Pleural fluid, amniotic fluid or pericardial fluid: 1 mL collected in a sterile leak-proof container
Whole blood: 1 mL collected in an EDTA (lavender-top) or ACD (yellow-top) tube
Bronchoalveolar lavage or bronichial wash collected in a sterile leak-proof container.
Vitreous fluid: 0.5 mL collected in a sterile leak-proof container
Minimum Volume
0.3 mL all other specimens • 0.35 vitreous fluid • 0.35 mL BAL or bronchial lavage/wash
Other Acceptable Specimens
Serum: 1 mL collected in an serum separator tube SST® or sterile tubes with no anticoagulants
Plasma: 1 mL collected in an EDTA (lavender-top) or ACD (yellow-top) or PPT (white-top) tube
Plasma: 1 mL collected in an EDTA (lavender-top) or ACD (yellow-top) or PPT (white-top) tube
Instructions
CSF: Collect in a sterile leak proof-container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in a viral transport media, VCM, M4 or APTIMA® Unisex Collection device (white label).
Bronchial Wash and BAL: Collect in a sterile leak-proof container.
Swab: Swab a lesion and place swab in a viral transport media, VCM, M4 or APTIMA® Unisex Collection device (white label).
Bronchial Wash and BAL: Collect in a sterile leak-proof container.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
| HSV 1 DNA | Not detected |
| HSV 2 DNA | Not detected |
Clinical Significance
DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the type.
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153
