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Direct bilirubin
MessageProtect from light or assay immediately. After centrifugation, if not in gel barrier tube, pour off plasma or serum into an aliquot tube.
Test Code
LAB52
Alias/See Also
Dbil, Conjugated Bilirubin
CPT Codes
82248
Preferred Specimen
Lithium Heparin (PST)
Minimum Volume
4.5 ml (600 ml min) lithium heparin (green); alternate-SST (gold) or Red (no additive)
Other Acceptable Specimens
Gold SST
Instructions
Spin PST or SST and send to lab
Transport Container
1 ml (200 ul minimum) plasma; alternate-serum
Transport Temperature
Room temp
Specimen Stability
Separated from cells: 4 hours room temp, if protected from light; 7 days refrigerated, if protected from light
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis: >10mg/dL; Lipemia: >300mg/dL
Methodology
Timed Endpoint Diazo Method
Setup Schedule
Mon-Sun
Reference Range
0.0-0.3 mg/dL
Clinical Significance
The assessment of direct bilirubin is helpful in the determination of hepatic disorders
Performing Laboratory
Piedmont Athens Regional