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Cortisol
MessageAfter centrifugation, if not in gel barrier tube, pour off serum into an aliquot tube.
Test Code
LAB61
CPT Codes
82533
Preferred Specimen
Lithium Heparin (PST)
Minimum Volume
4.5 ml (2.0 ml min) lithium heparin (green); alternate-SST (gold) or Red (no additive) tubes
Other Acceptable Specimens
Gold (SST)
Instructions
Spin PST and SST and send to lab
Transport Container
1 ml (500 ul minimum) plasma; alternate-serum
Transport Temperature
Room temp
Specimen Stability
Separated from cells: 8 hours room temp, 48 hours refrigerated
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Icteric: >10 mg/dL bilirubin, Hemolysis: >500 mg/dL hemoglobin, Lipemic: >1800 mg/dL triolein
Methodology
Immunoenzymatic
Setup Schedule
Mon-Sun
Reference Range
Cortisol A.M. – 6.7-22.6 μg/dL.
Cortisol P.M. - < 10 μg/dL. (10)
Cortisol P.M. - < 10 μg/dL. (10)
Clinical Significance
Abnormal changes in cortisol levels occur due to hypothalamic, pituitary, or adrenal malfunction. If undiagnosed
and untreated, these disorders can lead to severe metabolic imbalance which may be life-threatening
and untreated, these disorders can lead to severe metabolic imbalance which may be life-threatening
Performing Laboratory
Piedmont Athens Regional
