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Phenobarbital
Test CodeLAB30
Alias/See Also
Luminal
CPT Codes
80184
Preferred Specimen
4.5 ml (600 ul min) lithium heparin (green); alternate-SST (gold) or Red (no additive)
Minimum Volume
1 ml (200 ul minimum) plasma; alternate-serum
Other Acceptable Specimens
Gold SST
Instructions
After centrifugation, if not in gel barrier tube, pour off plasma or serum into an aliquot tube
Transport Temperature
Room Temp
Specimen Stability
Separated from cells: 48 hours room temp, 1 month refrigerated
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Icteric: >30 mg/dL bilirubin, Hemolysis: >800 mg/dL hemoglobin, Lipemia: >750 mg/dL, Amobarbital: >4 ug/mL, Mephobarbital: >5 ugl/mL
Methodology
Immunoassay
Setup Schedule
Mon- Sun
Reference Range
Therapeutic: 15.0 – 40.0 ug/mL
Toxic: >50.0 ug/mL
Toxic: >50.0 ug/mL
Clinical Significance
Monitoring serum phenobarbital concentrations, along with careful clinical assessment, is the most effective means
of improving seizure control, reducing the risk of toxicity, and minimizing the need for additional anticonvulsant
medication
of improving seizure control, reducing the risk of toxicity, and minimizing the need for additional anticonvulsant
medication
Performing Laboratory
Piedmont Athens Regional
