Lymphocyte Subset Panel 4 (NY)

Test Code
17331


CPT Codes
86360 (x2)<br><strong>This test code is for New York patient testing. For non-New York patient testing, use test code 7924.</strong>

Includes
% CD4, Absolute CD4+ Cells, % CD8, Absolute CD8+ Cells, CD4/CD8 Ratio, Absolute Lymphocytes


Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube


Minimum Volume
0.5 mL


Other Acceptable Specimens
Whole blood collected in: sodium heparin (green-top) tube


Instructions
Blood specimens should be maintained at room temperature during transport. For optimal results specimen should reach the laboratory within 24 hours of collection.

The change reflects the Center for Disease Control and Prevention (CDC) Guidelines and Recommendations for lymphocyte subsets performed by the single-platform method (CDC MMWR Weekly Report, January 31, 2003, Vol.52 No.RR2)


Transport Temperature
Room temperature


Specimen Stability
EDTA blood
Room temperature: 30 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Heparin blood
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lithium heparin (green-top) tube • ACD (yellow-top) tube • Samples beyond stability • Insufficient volume • Samples transported at >37° C • Received refrigerated • Received frozen


Methodology
Flow Cytometry (FC)

Setup Schedule
Set up: Daily; Report available: 2-3 days


Reference Range
See Laboratory Report


Clinical Significance
Assists in evaluating helper and suppressor cell immune status in immunodeficiency diseases such as AIDS.


Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.