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Vitamin D, 25-Hydroxy, Total, Immunoassay
Test Code17306
CPT Codes
82306
Preferred Specimen
0.8 mL serum
Patient Preparation
Fasting preferred, but not required.
Minimum Volume
0.5 mL
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Moderate hemolysis • Grossly lipemic • Plasma
Methodology
Immunoassay (IA)
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Reference Range
30-100 ng/mL
Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia
Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153