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HLA Typing – Transplant Workup
Test CodeARCHLA058
Alias/See Also
Performed at American Red Cross
HLA 058
HLA 058
CPT Codes
81378, 81382X5
Preferred Specimen
7.0 mL EDTA or ACD Whole Blood
Minimum Volume
2.0 mL EDTA or ACD Whole Blood
Other Acceptable Specimens
4 Buccal Swabs
Transport Container
Appropriate boxes or Bubble wrap on any container to avoid breakage of the sample tube during transportation. Swab samples should be kept in paper envelope, not in plastic bags to avoid mold.
Transport Temperature
Ambient or Refrigerated
Specimen Stability
Whole Blood Ambient: 72 hours
Whole Blood Refrigerated: 30 days
Buccal swab (dry) Ambient: 1 week
Buccal swab Frozen: 1 year
Whole Blood Refrigerated: 30 days
Buccal swab (dry) Ambient: 1 week
Buccal swab Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Incorrect labeling
Damaged sample tubes
Specimens collected in heparin
Damaged sample tubes
Specimens collected in heparin
Methodology
Next Generation Sequencing unless otherwise indicated on report; SBT for 24 hr TAT cases
FDA Status
Non-FDA Approved
Setup Schedule
Daily; Monday - Friday
Report Available
Reports in 3-4 days
Limitations
Rare typing errors can occur due to primer or probe site mutations. Reports are reported as a single field result.
