Alkaline Phosphatase, Bone Specific (APBONE)

Test Code

Quest Code

CPT Codes

Preferred Specimen
1 mL serum

Minimum Volume
0.3 mL

Allow serum samples to clot completely before centrifugation.

Transport Container
Preferred: Gold top; Acceptable: Plain red top

Transport Temperature
Room temperature

Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 21 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Non-serum samples • Grossly icteric • Gross lipemia

Immunoassay (IA)

Setup Schedule

Report Available
4 days

Reference Range
Adult Male 
18-29 years8.4-29.3 mcg/L
30-39 years7.7-21.3 mcg/L
40-49 years7.0-18.3 mcg/L
50-68 years7.6-14.9 mcg/L
>68 yearsNot established
Adult Female 
18-29 years4.7-17.8 mcg/L
30-39 years5.3-19.5 mcg/L
40-49 years5.0-18.8 mcg/L
50-76 years5.6-29.0 mcg/L
>76 yearsNot established
35-45 years5.0-18.2 mcg/L

2-24 months**25.4-124.025.4-124.0
25 months-5 yearsNot establishedNot established
6-9 years41.0-134.641.0-134.6
10-13 years43.8-177.424.2-154.2
14-17 years13.7-128.010.5-75.2
**Pediatric data from Int J Biol Markers 1996; 11:159-164.

Clinical Significance
The bone-specific alkaline phosphatase (BSAP) assay provides a general index of bone formation and a specific index of total osteoblast activity. BSAP and osteocalcin are the most effective markers of bone formation and are particularly useful for monitoring bone formation therapies and antiresorptive therapies.

Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA
14225 Newbrook Drive
Chantilly, VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.